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Journal of Medicinal Plants Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

P-ISSN: 2394-0530, E-ISSN: 2320-3862

2020, Vol. 8, Issue 1, Part A

GC-MS analysis and antibacterial activity of garlic extract with antibiotic

Rokhsana Mohamed Ismail, Antar Hamoud Ahmed Saleh and Khaled Saeed Ali

Background and Objective: Garlic (Allium sativum) is one of the herbs that used by traditional practitioners for preparation of herbals medicine. In the present study the antibacterial activity of aqueous and methanolic extracts of each local and imported garlic had been assayed separately against drug resistant clinical bacterial isolates include: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhi, in addition compare it's effect with conventional antibiotics.
Materials and Methods: The antibacterial activity was determined by disc and a gar well diffusion method. The analysis of local and imported garlic constituents were done by gas chromatography-mass spectrometry (GC-MS).
Results: The results showed that there were differences in the antibacterial effect of garlic types and each extract. The aqueous extracts were more potent especially local garlic than the methanolic extracts, and all combinations were inferior in activity, when compared to the standard Ampicillin and the two types of garlic individually. The Inhibition zone of garlic varied ranging diameters (9.6 – 23.7 mm). The minimum inhibitory concentration of different bacterial species varied from 0.048 g/ml to 0.768 g/ml. The GC-MS analysis indicated that GC-MS analysis of local aqueous garlic showed the presence of: 3-chlorothiophene [7.35%]; diallyldisulphide [13.93%];-3-vinyl-1,2-dithiacyclohex-4-ene[18.40%]; 3-vinyl1, 2 dithiacyclohex-5ene [4.12%]; ethyl trifluoro methyl trisulphide [1.78%]; amidino thiourea[1.04]. 3,3 ‟thiobis1-propene [3.34%]; 1,4-diathiane [2.75%]; N, N‟-dimethyl-thiourea [0.72%]; 3-hydroxy, methyl-esteroctadecanoic acid [1.34%]; 2-chlorobutoxyethylester acetic acid [2.73%]; and 2-methoxy-n-tetrahydro furfuryl acetamide [1.54%].
Conclusion: Garlic extracts showed a powerful inhibitory effect against pathogens compared with Ampicillin.
Pages : 26-30 | 1959 Views | 1006 Downloads

Journal of Medicinal Plants Studies
How to cite this article:
Rokhsana Mohamed Ismail, Antar Hamoud Ahmed Saleh, Khaled Saeed Ali. GC-MS analysis and antibacterial activity of garlic extract with antibiotic. J Med Plants Stud 2020;8(1):26-30.
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