Protective appraisal of Gynandropsis pentaphylla leaves against hepatic injury in rats
Vikram Singh and Dharmendra Singh
To appraise the protective effect of methanol extract of Gynandropsis pentaphylla leaves in carbon tetrachloride (CCl4)-induced hepatic injury in rats. The hepatic injury was induced in rats with the administration of a 1:1 (v/v) mixture of CCl4 in olive oil at the dose level of 1ml/kg intra-peritoneally once a week. The methanol extract of Gynandropsis pentaphylla (50 and 100 mg/kg) and the standard drug Silymarin (25 mg/kg) were given orally from 1 to day 15. The extract of Gynandropsis pentaphylla produced significant (p<0.001) dose-dependent protection on the altered hepatic-SOD (superoxide dismutase), CAT (catalase), GSH (glutathione reduced), GPx (glutathione peroxidase), LPO (lipid peroxidation) and cytochrome P-450 enzyme when compared with the toxic control. Serum was also analyzed for SGOT, SGPT (transaminases), ALP (alkaline phosphatase), GGT (gamma-glutamyl transpeptidase), LDH (lactate dehydrogenase), total bilirubin, and total protein levels. Gynandropsis pentaphylla extract produced a protective effect by decreasing the activities of serum enzymes, and total bilirubin along with increasing the total protein level when compared with toxic control. The results were compared with the standard drug silymarin. From the above results, it can be concluded that the observed hepatoprotective potential of Gynandropsis pentaphylla leaves might be due to the presence of tannins, alkaloids, terpenoids, steroids, total phenolics, and flavonoids in the extract and/or the purified compounds- Lupeol (I), β-sitosterol (II), α-amyrin (III), and β-amyrin (IV), which were isolated from the methanol extract of Gynandropsis pentaphylla leaves.