Synergistic anticancer effect of combining metformin with olive (Olea europaea L.) leaf crude extract on the human breast cancer cell line MCF-7
Rajy M Isleem, Mazen M Alzaharna and Fadel A Sharif
Breast cancer is one of the most prevalent cancers among women globally. Several in vitro studies have shown that Metformin and Olive (Olea europaea L.) Leaf extract have anticancer activity when applied separately. This study investigates the impact of combining Metformin and olive leaves extract on MCF-7 cancer cells. Extraction executed with Soxhlet using different solvents. Viability of MCF-7 cells following treatments were determined using MTT. Combinations were quantified using Chou and Talalay method. Expressions of TP53, BAD, BAX, CASP8, and Tristetraprolin were quantified using qPCR. Results show that various combination ratios elicited better antitumor effects on MCF-7 than singly. qPCR results show that Metformin acts through p53-independent apoptosis whereas Olive Leaf crude extract may have a different killing mechanism. In conclusion, the study shows for the first time that combining Olive Leaf extract with Metformin can significantly reduce viability of MCF-7 through a strong synergistic inhibitory effect.
Rajy M Isleem, Mazen M Alzaharna, Fadel A Sharif. Synergistic anticancer effect of combining metformin with olive (Olea europaea L.) leaf crude extract on the human breast cancer cell line MCF-7. J Med Plants Stud 2020;8(2):30-37.