in vitro mass scale propagation and seedlings development of medicinal orchid Dendrobium transparens Wall ex Lindl
Tapash Kumar Bhowmik and Md. Mahbubur Rahman
In the present investigation, in vitro seed germination and seedlings development of Dendrobium transparens Wall ex Lindl. Was assessed on agar solidified KC; MS; PM and MVW media with three different sources of carbohydrates viz. sucrose, glucose and lactose. PM medium proved to be best for early germination and PLBs formation followed by MS medium. Sucrose supplemented media was found to be best whereas lactose containing medium gave at least performance. Germinated PLBs were cultured on agar solidified or liquid MS and PM media supplemented with different concentrations and combinations of auxins, cytokinins. Highest shoot growth was found on liquid PM medium supplemented with 1.0 mg/l IAA and 1.0 mg/l BAP (3.58 ± 0.12 cm) followed by agar solidified PM + 1.0 mg/l NAA + 1.0 mg/l BAP (3.35 ± 0.13 cm). PM was found better than MS medium and liquid culture was superior than agar solidified condition for elongation of in vitro grown germinated seedlings. BAP, IAA was better in elongation media than Kn, NAA, Picloram. Agar solidified full strength MS with 1.0 mg/l IAA produced highest increased length and number of roots (3.27 ± 0.20 cm/shoot bud and 1.74 ± 0.13 no/shoot bud) followed by half strength hormone free MS medium (2.18 ± 0.15 cm/shoot bud and 1.56 ± 0.12 no/shoot bud). The well developed seedlings were acclimatized subsequently transferred to pots in green house and watered regularly.