2020, Vol. 8, Issue 5, Part B

author(s)
Tapash Kumar Bhowmik, Minhajur Rahman and Md. Mahbubur Rahman

abstract
In the present investigation, in vitro seed germination of Cymbidium aloifolium (L.) Sw. was assessed on 0.8% (w/v) agar solidified KC, MS, PM, VW media. Here, 3% (w/v) sucrose was applied in MS medium while KC, PM, VW media were prepared with 2% (w/v) sucrose. MS medium proved to be best (93.34%) for early germination and greenish PLBs formation followed by PM (86.87%), VW (73.34%) and KC (73.34%) media. Minimum time needed for in vitro germination on MS (9.33±0.33^a weeks) medium followed by PM (10.47±0.31^b weeks), VW (11.34±0.31^c weeks) and KC (12.37±0.26^d weeks) media. However, the percentage of seed germination is equal in KC and VW media but the time is required minimum in VW compared to KC medium. The well-developed rooted seedlings were acclimatized and subsequently transferred to pots in green house with regular watering. Comparative exploration of secondary metabolites were done using in vitro callus, shoot buds, SPSs and leaf, root, stem sample collected from the nature. Natural leaf, root, stem sample of Cymbidium aloifolium exhibited the presence of maximum metabolites followed by in vitro developed SPSs, callus and shoot buds.