Assessment of toxicologic and radical scavenging potentials of methanol extract of ageratumconyzoides
Chinenye Azuka Mere, Jonathan Chinenye Ifemeje, Kizito Ifeanyi Amaefule and Andrew Chibuzo Nwaka
Aims:Ageratum conyzoides is an annual herbaceous plant commonly used in African traditional medicine as a purgative, antipyretic and wound dressing agent. The objective of this study was to investigate the free radical scavenging and toxicologic potentials of methanol extract of A.conzoides leaves in wistar rats.
Methods: The ground plant leaves were macerated to prepare a methanol extract and the ability of the extract to scavenge DPPH and reduce Fe3+ to Fe2+ was assayed following standard methods. The acute toxicity test was done using eighteen (18) male albino mice. The limit test dose of 5000 mg/kg body weight (b.w.) was administered to the albino mice and then observed individually 24 hrs post-dosing. Sub-chronic toxicity was evaluated after administering daily oral doses of 100, 200 and 400 mg/kg (b.w.), for 28 days using thirty two (32) rats. The effect of the extract on liver and kidney functions were assessed following standard methods.
Results: The extract scavenged DPPH in a dose dependent manner with EC50 of 3.88 µg/ml compared to ascorbic acid EC50 of 2.37 µg/ml also reduced Fe3+ to Fe2+ in a concentration dependent form. The limit dose of 5000 mg/kg did not cause any mortality or signs of acute toxicity in the rats tested during the observation period. In the sub-chronic tests, the results did not show any treatment–related abnormalities in biochemical parameters. However, urea was significantly (p<0.05) lower in the group treated with 500 mg/kg of A. conyzoides extract.
Conclusion: The results suggest that the methanol extract of A. conyzoides is relatively safe when administered orally in rats and possesses free radical scavenging properties.
Chinenye Azuka Mere, Jonathan Chinenye Ifemeje, Kizito Ifeanyi Amaefule, Andrew Chibuzo Nwaka. Assessment of toxicologic and radical scavenging potentials of methanol extract of ageratum conyzoides. J Med Plants Stud 2021;9(2):01-05.