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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862

Journal of Medicinal Plants Studies

2022, Vol. 10, Issue 1, Part A
Growth and secondary metabolites production of Kaempferia galanga L. Callus under the influence of ultraviolet: B radiation

Shofiyani Anis, Suwarto, Suprayogi and Yuniati Alice

Kaempferia galanga L. is one of the herbal plants that has been used for generations by the community because it has medicinal properties. The development of this plant continues to decline due to the conversion of land for food crops which results in the decreasing supply of quality herbal raw materials for the pharmaceutical and chemical industries. A promising alternative for developing medicinal plants capable of producing secondary metabolites without competing with land use for food crops is in vitro culture, especially callus culture. This study aimed to determine the effect of UV-B radiation on the growth, secondary metabolite production, and antioxidant capacity of K. galanga callus. The treatments included the intensity of ultraviolet-B radiation and radiation exposure time. The intensity of UV radiation with three levels, namely 70, 140, and 210 µW/cm2, UV-B radiation exposure time was 2, 4, and 6 hours. Phytochemical parameters of UV-B radiation treatment had no significant effect on total phenol content. The combination of treatment intensity and duration of exposure affected the parameters of total flavonoid levels in K. galanga callus during the study, namely the combination of treatment intensity 140 µW/cm2 and UV-B radiation exposure time of 2 hours, namely 53.94 ± 4.19 µg/g DW callus. At 2 hours of exposure to UV-B radiation, antioxidant activity showed the highest antioxidant ability, 49.51 ± 4.23%. PAL enzyme activity did not show any effect of UV-B elicitation on PAL enzyme activity to increase the content of phenolic compounds. GCMS test results showed that exposure to UV-B irradiation induces several compounds that have medicinal functions.
Pages : 01-07 | 204 Views | 93 Downloads
How to cite this article:
Shofiyani Anis, Suwarto, Suprayogi, Yuniati Alice. Growth and secondary metabolites production of Kaempferia galanga L. Callus under the influence of ultraviolet: B radiation. J Med Plants Stud 2022;10(1):01-07.
Journal of Medicinal Plants Studies
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