2022, Vol. 10, Issue 1, Part C

Isoenzyme studies were carried out in these morphotypes to reveal genetic relationships among them. Five enzyme systems, Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Peroxidase (Per), Acid phosphatase (ACP) and Leucine amino peptidase (LAP) were studied for this purpose using native-Page. Out of these, only three enzyme systems (EST, GOT, PER) produced reproducible and consistent bands and were further analyzed. The analysis of eight isozyme loci revealed that these morphotypes exhibit low genetic variability (P=15.1%, A=1.15, Ho=0.015, He=0.088). Two morphotypes (pinkish white and white) have negative fixation index values i.e. FIS = -1 which showed more heterozygosity than observed. The pink flowered morphotype showed more observed heterozygosity than expected, and hence having FIS = +1. The dendrogram constructed based on cluster analysis (UPGMA) suggested that morphotypes; pinkish white and white were closely related (63% similarity). While pink flowered morphotype showed only 26% similarity and was distantly related to others.