Contact: +91-9711224068
Journal of Medicinal Plants Studies
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

P-ISSN: 2394-0530, E-ISSN: 2320-3862

2022, Vol. 10, Issue 1, Part C

Isoenzyme studies in a polymorphic orchid species, Satyrium nepalense D. Don

Kusum, Verma Jagdeep and Sembi Jaspreet Kaur

Isoenzyme studies were carried out in these morphotypes to reveal genetic relationships among them. Five enzyme systems, Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Peroxidase (Per), Acid phosphatase (ACP) and Leucine amino peptidase (LAP) were studied for this purpose using native-Page. Out of these, only three enzyme systems (EST, GOT, PER) produced reproducible and consistent bands and were further analyzed. The analysis of eight isozyme loci revealed that these morphotypes exhibit low genetic variability (P=15.1%, A=1.15, Ho=0.015, He=0.088). Two morphotypes (pinkish white and white) have negative fixation index values i.e. FIS = -1 which showed more heterozygosity than observed. The pink flowered morphotype showed more observed heterozygosity than expected, and hence having FIS = +1. The dendrogram constructed based on cluster analysis (UPGMA) suggested that morphotypes; pinkish white and white were closely related (63% similarity). While pink flowered morphotype showed only 26% similarity and was distantly related to others.
Pages : 215-221 | 253 Views | 117 Downloads
How to cite this article:
Kusum, Verma Jagdeep, Sembi Jaspreet Kaur. Isoenzyme studies in a polymorphic orchid species, Satyrium nepalense D. Don. J Med Plants Stud 2022;10(1):215-221.
Call for book chapter
Journal of Medicinal Plants Studies
Please use another browser.