Embryogenesis of Dipterocarpus retusus Bl. Syn. D. macrocarpus
Papori Phukan Borpuzari and Jonaki Kachari
Somatic embryogenesis was achieved in tree species Dipterocarpus retusus using tender leaf and petiole explants on Murashige and Skoog (MS) medium supplemented with 2, 4-D 2.0+IAA 0.5mg/l and 3% sucrose. MS medium containing 2, 4-D 4.0mg/l was recorded to be the most effective on inducing of embryonic callus. Embryogenesis per culture was varied in MS medium supplemented in different concentration in 2, 4-Dmg/l. Maximum number of somatic embryos was obtained in MS medium containing 2, 4-D 4.0mg/l and proliferation of embryonic callus was enhanced in the cultures having 2, 4-D 2.0mg/l in reducing of 2, 4-D in the media. Somatic embryos in various shapes and sizes after the first subculture on MS medium supplemented with 2,4-D 4.0mg/l. Embryo germination and was observed in the ½ MS medium and optimal results was attained in ½ MS + GA3 0.1 with leafy cotyledon development with distinct root and shoot pole.