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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862

Journal of Medicinal Plants Studies

2022, Vol. 10, Issue 4, Part A
Embryogenesis of Dipterocarpus retusus Bl. Syn. D. macrocarpus

Papori Phukan Borpuzari and Jonaki Kachari

Somatic embryogenesis was achieved in tree species Dipterocarpus retusus using tender leaf and petiole explants on Murashige and Skoog (MS) medium supplemented with 2, 4-D 2.0+IAA 0.5mg/l and 3% sucrose. MS medium containing 2, 4-D 4.0mg/l was recorded to be the most effective on inducing of embryonic callus. Embryogenesis per culture was varied in MS medium supplemented in different concentration in 2, 4-Dmg/l. Maximum number of somatic embryos was obtained in MS medium containing 2, 4-D 4.0mg/l and proliferation of embryonic callus was enhanced in the cultures having 2, 4-D 2.0mg/l in reducing of 2, 4-D in the media. Somatic embryos in various shapes and sizes after the first subculture on MS medium supplemented with 2,4-D 4.0mg/l. Embryo germination and was observed in the ½ MS medium and optimal results was attained in ½ MS + GA3 0.1 with leafy cotyledon development with distinct root and shoot pole.
Pages : 38-45 | 22 Views | 8 Downloads
How to cite this article:
Papori Phukan Borpuzari, Jonaki Kachari. Embryogenesis of Dipterocarpus retusus Bl. Syn. D. macrocarpus. J Med Plants Stud 2022;10(4):38-45.
Journal of Medicinal Plants Studies
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