Preliminary phytochemical analysis and antimicrobial activity of leaves of Barleria acanthoids Vahl
Dr. Nidhi Shukla and Dr. Bramhanand Shukla
Present investigation deals with the preliminary phytochemical analysis and antibacterial activity of Barleria acanthoids. The leaves of this plant was subjected to successive Soxhlet extraction using petroleum ether, chloroform and ethanol. The various leaf extracts, were subjected to preliminary phytochemical screening for different classes of phyto constituents. Phytochemical analysis, ethanol extracts showed presence of all compounds. The three different concentrations range from 30, 60 and 90 µl /ml of the extracts were tested for antibacterial activity using agar disc diffusion assay method against Salmonella typhi, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Streptomycin was used as a reference drug. The ethanolic extract exhibit highest zone of inhibition against Staphylococcus aureus is 36 mm with 90 µl /ml concentration and followed by Streptococcus pyogenes.