2022, Vol. 10, Issue 5, Part B

author(s)
Digambar Subhashrao Pawar and Jyoti Udhavrao Ghodke

abstract
Medicinal plants contain number of important secondary metabolites which are able to control fungal diseases. Even it is safer and alternative to synthetic fungicides. As we all know, chemical fungicides are harmful not only to humans but also to animals, other vegetation, and the entire ecosystem, so we must focus on other alternatives that are biologically safe and nonhazardous. In the present investigation, aqueous extract of leaf of Madhuca indica and Eucalyptus alba which were found antifungal against Phytophthora infestans were exposed to various physical factors such as Autoclave, Temperature, pH, Humidity etc. The exposed extracts were tested against given fungi. The effect of physical factors were investigated by subjecting the extract to varying circumstances of the parameters chosen for a specified time period and then observing the effect as a function of change in the extract's MIC against the test organism. Experiments were repeated thrice and three replicates were maintained besides control. Result suggested that efficacy of leaf extracts against tested fungus were slightly affected by various physical factors. Hence from the results we finding autoclaving, high temperature, alkaline pH and high humidity decrease the antifungal activity of plant extracts.