To prepare cantharis glycerol at different ratio with UV visible spectrophotometer and FTIR
Dr. Suraj Singh Bhadoria, Chauhan Hareshsinh Harjibhai, Parmar Vasant kumar Ganpat Sinh, Dr. Monimala Pramanick, Dr. PS Mandal, Dr. Mayank Roy, Dr. GS Chakroborthy, Dr. Poorav Desai and Tejas Kumar Bhatt
Background: Through this research work preparing formulation of the glycerol by the standard cantharis mother tincture in Drug and vehicle ratio of (1:1), (1:4) and (1:9) under the analysis by UV- visible spectrophotometer and FTIR.
Methodology: The formulation of glycerol is prepared with Cantharis mother tincture divided into three groups such as; Standard group with Cantharis mother tincture, Main sample with Cantharis glycerol in different drug and vehicle ratio and control group with glycerol. These samples were taken in the cuvette around 3-4 ml placed inside the UV chamber to measured their absorbance whereas in FTIR a one drop of each samples was placed over the lens to measured the Maximum transmission of the light.
Results: The analysis done by UV visible spectrophotometer shows absorbance of Cantharis mother tincture is 0.998 at 433 nm, Glycerol is 0.741 at 278 nm, Cantharis glycerol (1:9) is 0.987 at 333 nm, Cantharis glycerol (1:4) is 0.997 at 368 nm and Cantharis glycerol (1:1) is 0.996 at 367 nm. Whereas in FTIR the maximum transmission of Cantharis Q is 1045.35 cm-1, Glycerol is 1032.40 cm-1, Cantharis glycerol (1:9) is 1033.13 cm-1, Cantharis glycerol (1:4) is 1033.82 cm-1 and Cantharis glycerol (1:1) is 1033.93 cm-1.
Dr. Suraj Singh Bhadoria, Chauhan Hareshsinh Harjibhai, Parmar Vasant kumar Ganpat Sinh, Dr. Monimala Pramanick, Dr. PS Mandal, Dr. Mayank Roy, Dr. GS Chakroborthy, Dr. Poorav Desai, Tejas Kumar Bhatt. To prepare cantharis glycerol at different ratio with UV visible spectrophotometer and FTIR. J Med Plants Stud 2023;11(1):135-139.