2023, Vol. 11, Issue 1, Part B

Background: Through this research work preparing formulation of the glycerol by the standard cantharis mother tincture in Drug and vehicle ratio of (1:1), (1:4) and (1:9) under the analysis by UV- visible spectrophotometer and FTIR.

Methodology: The formulation of glycerol is prepared with Cantharis mother tincture divided into three groups such as; Standard group with Cantharis mother tincture, Main sample with Cantharis glycerol in different drug and vehicle ratio and control group with glycerol. These samples were taken in the cuvette around 3-4 ml placed inside the UV chamber to measured their absorbance whereas in FTIR a one drop of each samples was placed over the lens to measured the Maximum transmission of the light.

Results: The analysis done by UV visible spectrophotometer shows absorbance of Cantharis mother tincture is 0.998 at 433 nm, Glycerol is 0.741 at 278 nm, Cantharis glycerol (1:9) is 0.987 at 333 nm, Cantharis glycerol (1:4) is 0.997 at 368 nm and Cantharis glycerol (1:1) is 0.996 at 367 nm. Whereas in FTIR the maximum transmission of Cantharis Q is 1045.35 cm^-1, Glycerol is 1032.40 cm^-1, Cantharis glycerol (1:9) is 1033.13 cm^-1, Cantharis glycerol (1:4) is 1033.82 cm^-1 and Cantharis glycerol (1:1) is 1033.93 cm^-1.