2023, Vol. 11, Issue 1, Part C

author(s)
Neha Kisku and Akshay Kumar Verma

abstract
The present study aimed to identify and characterize the phytochemical constituents of the methanolic extract of Blepharis integrifolia using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The chromatogram revealed 30 bioactive compounds with retention times ranging from 14.27 to 32.13 minutes. The major constituents identified were n-Hexadecanoic acid (16.56%), 9, 12, 15-Octadecatrienoic acid (12.46%), 8, 11, 14-Docosatrienoic acid, methyl ester (9.07%), 11, 14-Eicosadienoic acid (6.31%), and Stigmasterol (4.64%). Other notable compounds included Vitamin E, Squalene, Phytol, and Gamma-sitosterol, which are known for their antioxidant, anti-inflammatory, and antimicrobial properties. The presence of diverse fatty acids, sterols, and terpenoids suggests the pharmacological potential of B. integrifolia as a source of natural bioactive molecules.