Comparative phytochemical and antioxidant activity of Trewia nudiflora leaf and fruit
Mohammad Mostafa Mohsin Milon, Ranjan Kumar Sarker, Razia Sultana and GM Masud Parvez
The main intention of the study was comparative phytochemical and antioxidant activity screening of leaf, peel and flesh parts of the medicinal plant Trewia nudiflora in terms of their phenolic content, flavonoid content, total antioxidant capacity, reducing power capacity and radical scavenging capacity. The leaf, peel and flesh were dried and grinded to course powder and extracted with methanol and ethyl acetate solvents. Among methanol and ethyl acetate extracts, methanolic extract of leaf of Trewia nudiflora (LTNM) carried the maximal amount of phenolic content 14.537±0.696 mg of GAEs/gm and ethyl acetate extract of flesh of T. nudiflora (FTNE) comprised the most amount of phenolic content 14.298±0.680 mg of GAEs/gm. In our study, among all the extracts, methanolic extract of leaf of Trewia nudiflora (LTNM) possessed maximum amount of flavonoid content 5.398±0.414 mg of CAEs/gm. It was observed that the leaf of Trewia nudiflora (LTNM) contained the highest amount of antioxidant capacity 29.311±0.429 mg of AAE/g. Among all the extracts, leaf of Trewia nudiflora (LTNM) signified the greatest reducing power capacity. Among all the methanolic extract the leaf (LTNM) showed the utmost scavenging activity 72.517% that was the closest to the standard BHT 83.427%. The flesh (FTNE) showed the most scavenging activity (70.909%) among all ethyl acetate extracts. In our study, the IC50 value ranged from 40.95 to 172 μg/ml except the ethyl acetate extract of leaf. This indicates significant radical scavenging activity. Finally, the leaf extract had given the most significant phytochemical and antioxidant activity in all the cases.