2023, Vol. 11, Issue 5, Part A

author(s)
PM Purohit

abstract
Profuse somatic embryoids were obtained from leaf segments cultured on MS media supplemented with IAA (0-1.5 mg/l) and 2, 4-D (0-1.5 mg/l) after 80 days in no light condition. The leaf segments were collected from in vitro shoot multiplication cultures. Embryoids were cultured on MS media supplemented with BA (0.5-2.0 mg/l) and 2, 4-D (0-1.0 mg/l) for development and maturation. Different shaped embryos were developed after four weeks. Mature embryos developed into shoots and roots when placed on hormone free half strength MS media, after 6 weeks.