GC-MS analysis and quantitative phytochemical analysis of Phyllanthus reticulatus Poir
Saima PM, Aleema Sherin, Anitta Thimothy, Jabina Sherin, Shibla KK and Sinija Shirin
Phyllanthus reticulatus Poir is a medically important plant in the family Euphorbiaceae. Traditionally they are utilized for their various medicinal properties. The present study deals with the GC-MS analysis and quantitative phytochemical analysis of ethanolic extract of Phyllanthus reticulatus poir. By GC-MS analysis, thirty compounds including Pyranone, dodecane, guanosine, loliolide, and vitamin E were identified. Quantitative phytochemical analysis for alkaloids, flavonoids, and phenol was performed, and the total alkaloid content was determined using colorimetry using atropine as standard and was found to be 96.48 µg/ml. The total flavonoid content of ethanolic extract was determined using an Aluminium chloride colorimetric assay by comparing it with the standard absorbance curve of quercetin was found to be 171.71 µg/ml. The total phenolic content of ethanolic extract was determined using the Folin- Ciocalteu method by using standard absorbance cure of Gallic acid and was found to be 131.39µg/ml.