2024, Vol. 12, Issue 5, Part A

author(s)
Vaishnavi Mali, Vaishnavi Patil1, Samradnyee Sawant, Shital Devkar, Prachi Khochage and Dr. NB Chougule

abstract
This study focuses on the formulation and evaluation of an antimicrobial polyherbal gel utilizing extracts from Acacia nilotica and Ocimum sanctum plants. With the escalating threat of antimicrobial resistance, there is an urgent need for novel therapeutic agents, particularly those derived from natural sources. The objective of this research is to develop a topical gel formulation that harnesses the combined antimicrobial potential of Acacia nilotica Bark extract and Ocimum sanctum leaf extracts for potential applications in wound healing and dermatological infections. The formulation process involved the extraction of bioactive compounds from Acacia nilotica and Ocimum sanctum leaves and bark using suitable solvents. These extracts were then incorporated into a gel base following standard procedures. The resulting polyherbal gel was characterized for its physical properties, including appearance, pH, spreadability, and viscosity, to ensure suitability for topical application. The antimicrobial activity of the polyherbal gel was evaluated against a panel of pathogenic microorganisms, including both Gram-positive and Gram-negative bacteria, as well as fungi. The agar well diffusion method and broth microdilution assay were employed to determine the inhibitory effects of the gel against the test microorganisms. The results revealed significant antimicrobial activity, with notable inhibition zones and minimum inhibitory concentrations against the tested pathogens. Furthermore, the cytotoxicity of the polyherbal gel was assessed using in vitro cell viability assays to ensure its safety for topical use. The results demonstrated negligible cytotoxic effects on mammalian cells, highlighting the potential biocompatibility of the formulation. In conclusion, the formulation and evaluation of the antimicrobial polyherbal gel utilizing Acacia nilotica and Ocimum sanctum extracts offer a promising strategy for the development of natural antimicrobial agents. The synergistic action of these plant extracts provides a valuable alternative to synthetic antibiotics, contributing to the ongoing efforts in combating antimicrobial resistance and promoting sustainable healthcare solutions.