Synergistic defense: Formulation and development of polyherbal topical approach for enhanced protection
Synergistic defense: Formulation and development of polyherbal topical approach for enhanced protection
In light of the escalating global health threat posed by antibiotic resistance, the search for alternative antimicrobial agents has become paramount. This study presents the formulation and development of a novel herbal antibacterial gel utilizing extracts derived from Vitex negundo leaves and Vachellia Nilotica bark. The gel formulation was meticulously crafted employing a blend of traditional extraction techniques and modern pharmaceutical principles to ensure the optimal extraction and preservation of bioactive constituents. Various physicochemical parameters including pH, viscosity, spreadability, and stability were systematically optimized to achieve an ideal formulation with desirable characteristics for topical application. Subsequently, the antibacterial efficacy of the formulated gel was extensively evaluated against a spectrum of clinically relevant bacterial strains, encompassing both Gram-positive and Gram-negative organisms. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays were employed to determine the potency of the gel against bacterial growth. Our results demonstrate that the herbal antibacterial gel exhibited remarkable inhibitory activity against all tested bacterial isolates, including multidrug-resistant strains. Furthermore, the gel displayed dose-dependent bactericidal effects, with higher concentrations yielding enhanced antimicrobial efficacy. Mechanistic studies elucidated the underlying modes of action, revealing a multifaceted approach involving disruption of bacterial cell membranes, inhibition of essential metabolic pathways, and interference with bacterial cell wall synthesis. Moreover, the developed herbal gel demonstrated favorable physical characteristics, including smooth texture, good spreadability, and long-term stability, rendering it suitable for topical application. Overall, this study underscores the promising potential of Vitex negundo leaves and Vachellia Nilotica bark extracts as effective antibacterial agents in the formulation of herbal gels. The successful development of this herbal antibacterial gel holds significant implications for the management of bacterial infections, offering a safe, natural, and sustainable alternative to conventional antibiotics. Further research is warranted to explore its clinical efficacy and safety profile for therapeutic applications.
Synergistic defense: Formulation, development of polyherbal topical approach for enhanced protection. Synergistic defense: Formulation and development of polyherbal topical approach for enhanced protection. J Med Plants Stud 2024;12(5):32-42.