2025, Vol. 13, Issue 3, Part C

author(s)
Smrutiranjan Dash, Rajasekaran S, Nawed Alam and Soumya Priyadarshi Behera

abstract
Urochloa distachya
is a perennial grass belonging to the Poaceae family. This study aimed to
identify the functional groups present in U. distachya using
Fourier-transform infrared (FTIR) spectroscopy. Plant samples were shade-dried,
powdered, and subjected to Soxhlet extraction using petroleum ether,
chloroform, ethyl acetate, and ethanol. FTIR analysis was conducted with a
Bruker 3000 Hyperion system using OPUS software. The spectra revealed key
functional groups, including hydroxyl, carbonyl, aliphatic, and aromatic
moieties, indicating the presence of phenolics, flavonoids, esters, and fatty
acids. Notable variations in peak intensity were observed across the U.
distachya powder and its extracts. The FTIR absorption ranges were: powder
(657.59-3901.13 cm^-1), petroleum ether extract (PUD) (1377.38-2917.59
cm^-1), chloroform extract (CUD) (754.01-2916.42 cm^-1),
ethyl acetate extract (EAUD) (1042.85-1736.25 cm^-1), and ethanol
extract (EUD) (878.99-3352.21 cm^-1). Overall, the FTIR results
confirm the phytochemical richness of U. distachya and highlight its
potential for further pharmacological and bioactive compound investigations.