Plants served as a significant source of traditional medicine used to cure human illness. Numerous plant nutritional benefits have been discussed in literature, but there is relatively very limited research that has been done on biologically active chemicals present in Trema orientalis. With the help of the Soxhlet apparatus, the extraction of sample from the bark and leaf of Trema orientalis was done. Distilled water, methanol and ethanol were used as solvents. After the phytochemical screening of the extract, we found the test positive for glycoside, saponin, tannin, coumarin, terpenoids, and phenol, which implies their presence in Trema orientalis. These are the non-nutritive parts that have inhibitory properties against neurological disorder, microbes, malaria, cancer and inflammation etc. It is traditionally used for the prevention of cough, fever, diarrhea, toothache, and pneumonia and for different digestive issues. The wood of Trema orientalis was traditionally used in the making gunpowder. In view of its rare occurrence in Western Odisha, it holds a great promise for further research for identifying bioactive molecules that can be used as novel medicines