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Journal of Medicinal Plants Studies
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P-ISSN: 2394-0530, E-ISSN: 2320-3862

Peer Reviewed Journal

2025, Vol. 13, Issue 3, Part D

Isolation, Characterization and quantification of gallic acid from hydro alcoholic flower extract of Woodfordia fruticosa (L.) kurz along with fingerprinting analysis by HPTLC, HPLC, FT-IR, and NMR (1 H and 13C) spectroscopy

Ganesh Dey, Nikita Ghosh, Sushilta Prodhan, Simran Giri and Bapi Ray Sarkar

Ethnopharmacological knowledge of the plant Woodfordia fruticosa (L.) Kurz. is very precious in traditional system of medicine. Gallic Acid (3,4,5-trihydroxybenzoic acid) is one of the most abundant phenolic acids of the flower extract of this plant. Gallic acid (GA) shows a broad range of beneficial effects in prevention and treatment of several diseases. The pharmacological activities of GA are antioxidant, anti-diabetic, antimicrobial, anti-inflammatory, cardioprotective, anticancer, neuroprotective and gastroprotective. The research deals with isolation and quantification of GA by using Column Chromatography and HPLC. The abundant of Gallic acid (GA) was come out 2.28µg/mg in the WFF extract. The FT-IR spectrum analysed the presence of carboxylic group (2700-3600 cm-1), hydroxyl phenolic groups (3284, 3382 cm-1), carbonyl group (1705 cm-1) and an aromatic moiety (1541, 1618 cm-1). 1H-NMR and 13C- NMR spectrum of Gallic Acid (GA) was successfully recorded. So the hydro alcholic extract of WFF confirm the presence of Gallic Acid.
Pages : 318-323 | 1133 Views | 310 Downloads


Journal of Medicinal Plants Studies
How to cite this article:
Ganesh Dey, Nikita Ghosh, Sushilta Prodhan, Simran Giri, Bapi Ray Sarkar. Isolation, Characterization and quantification of gallic acid from hydro alcoholic flower extract of Woodfordia fruticosa (L.) kurz along with fingerprinting analysis by HPTLC, HPLC, FT-IR, and NMR (1 H and 13C) spectroscopy. J Med Plants Stud 2025;13(3):318-323. DOI: 10.22271/plants.2025.v13.i3d.1875
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