2025, Vol. 13, Issue 4, Part C

author(s)
Ayesha Khatoon, Rishita Mathuria, Muskan Kumari and Vinod Kumar Gupta

abstract
This study explores the antioxidant potential of various fruits using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to better understand how fruit-based antioxidants contribute to human health. Antioxidants are vital in neutralizing free radicals that can lead to chronic conditions such as heart disease, cancer, neurodegenerative disorders, and inflammation. The DPPH method is commonly used because it is both simple and effective in measuring antioxidant capacity. It works by observing changes in absorbance at 517 nm as antioxidants interact with DPPH radicals. Results revealed that Mangifera indica (mango) showed the highest antioxidant activity at around 95%. Other fruits, including Actinidia deliciosa (kiwi), Carica papaya (papaya), Prunus domestica (plum), Syzygium cumini (jamun), Malus pumila (apple), Musa acuminata (banana), and Citrus limetta (sweet lime), also demonstrated high antioxidant levels above 90%. These findings reinforce the importance of fruit-rich diets and support further research into natural antioxidants and their role in disease prevention.