2025, Vol. 13, Issue 5, Part A

author(s)
Prajapati Dimpal and Patel Ashok Kumar

abstract
Leptadenia reticulata (Retz.) Wight & Arn., an endangered medicinal plant known for its therapeutic properties in traditional Ayurvedic medicine, faces rapid depletion in the wild due to overharvesting and habitat loss. The present study establishes an efficient in vitro regeneration protocol using nodal explants cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxins. Maximum shoot induction (90% frequency) was observed on MS medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) + 0.15 mg/l NAA, resulting in an average of 7.6 ± 0.6 shoots per explant. The elongated shoots rooted best on half-strength MS medium containing 1.0 mg/L indole-3-butyric acid (IBA) and 0.5 mg/L ?-naphthalene acetic acid (NAA), yielding a 90% rooting frequency and 7.6± 0.4 roots per shoot. The plantlets were successfully acclimatized in a greenhouse with an 85% survival rate. This protocol offers a reliable method for ex situ conservation and large-scale propagation of L. reticulata.