2025, Vol. 13, Issue 5, Part B

author(s)
Andrew Kwaji, Wilson L Danbature and Ibrahim M Umar

abstract
The study was aimed at isolation of bioactive compounds from Chrozophora senegalensis, traditionally used for treating ailments. Plant sample was extracted with methanol using maceration and fractionated with solvents of varying polarity. Fractions were screened for antibacterial activities against clinical isolates. The most active ethylacetate fraction was evaluated for antioxidant potentials and purified on column chromatography. The antibacterial potential was significant relative to the standard with inhibition zones ranging from 10.43 - 21.53 mm against Gram-positive and Gram-negative bacteria. Antioxidant activity evaluation yielded fifty percent inhibitory concentrations (IC₅₀) ranging from 6.30 - 7.46 mg/mL while that of quercetin standard ranged from 5.01 - 8.71 mg/mL using standard procedures. The purification effort yielded quercetin and ?-sitosterol. The two compounds are known to possess antioxidant and antibacterial properties. Consequently, the isolation of these phytomolecules from Chrozophora senegalensis crude extract partly justifies its use in traditional medicine.