2025, Vol. 13, Issue 5, Part B

author(s)
Praveen T and Siddappa B Kakkalameli

abstract
The present investigation deliberates the chemical profiling and biological activities of Cynarospermum asperrimum (Nees) Vollesen. Using three different polar and non-polar solvents. The proximate analysis reveals the presence of various components, including total carbohydrates, total protein, and total lipids. The qualitative and quantitative phytochemical analysis reveals that it is rich in Phytoconstituents, showing the presence of polyphenols, alkaloids, flavonoids, and tannins, among others. The GC-MS analysis shows significant bioactive compounds linked to multiple health benefits, The FTIR analysis shows functional groups based on peak values, the peaks are detected from 4000cm^-1 to 600cm^-1. The antibacterial activity (Escherichia coli and Staphylococcus aureus) and Antifungal activity (Aspergillus flavus and Pichia anomala) show significant zones of inhibition. Antioxidant assay by the DPPH method reveals that the IC50 value is 57.24±3.83 µg/ml in the methanol extract, due to the presence of various antioxidant phytochemicals, indicating the potential for scavenging free radicals. Distilled water, and chloroform show moderate results. The in vitro Cytotoxicity by MTT assay against MCF-7 and HepG2 cell lines both show significant results with IC₅₀ values of MCF-7 58.38±3.13 µg/ml, and HepG2 67.57±4.1 µg/ml, in the methanol extract. These findings suggest Cynarospermum asperrimum (Nees) Vollesen. Leaf has promising functional pharmaceutical applications.