2025, Vol. 13, Issue 5, Part C

author(s)
Nidhi Anand and Rajesh Kumar

abstract
Hibiscus rosa-sinensis is a widely available plant in India, traditionally recognized for its medicinal properties. This study evaluated the antifertility efficacy of its aqueous flower extract in male Swiss albino mice (Mus musculus). 60 adult male mice were divided into two main groups: Group I (300 mg/kg body weight) and Group II (500 mg/kg body weight). Each group was further subdivided into control and treated group. Control group were fed orally 0.1 ml of glass distilled water, while treated groups were administered 0.1 ml of H. rosa-sinensis flower extract orally for 15, 30, 45, and 60 days. Mice were sacrificed and semen samples were obtained from both the cauda epididymis. Seminal parameters like sperm count, sperm motility, sperm mortality, sperm abnormality and seminal pH were assessed. Statistical analysis was done using student t-test. At 300 mg/kg body weight, treated group of mice showed a significant reduction (p<0.01) in sperm count, motility, and seminal pH, while increase in sperm mortality and abnormalities (p<0.01). At 500 mg/kg body weight, these effects were highly significant (p<0.001), indicating a dose-dependent antifertility action. The aqueous flower extract of H. rosa-sinensis exerts strong, dose-dependent antifertility effects in male mice and may serve as a safe, reversible, and economical herbal contraceptive for males.