2025, Vol. 13, Issue 5, Part C

author(s)
Dipan Sarma and Badal Kumar Datta

abstract
In this study, Aphanomixic polystachya leaves were used to quantify secondary metabolites and evaluated for in vitro antioxidant activity. The higher phenolic constituents were estimated in the aqueous extract (14.8±0.2 mg GAE/g) than in the methanol and acetone extracts. The methanol extract contained more tannins (6.81±0.14 mg GAE/g) and higher flavonoid content (15.45±0.1mg RE/g), whereas the acetone extract contained higher saponin (25.72±0.04 mg DE/g). The estimated vitamin C was found to be 3.76±0.05 mg AAE/g. Methanol extract exhibits robust DPPH scavenging activity with an IC₅₀ value of 57.85±1.73 ?g/mL, and all extract exhibits moderate H₂O₂ radical scavenging activity: methanol (108.08±1.88 ?g/mL), aqueous (177.24±4.35 ?g/mL), and acetone (224.60±3.66 ?g/mL). Acetone extract showed the highest chelating activity with an IC₅₀ value (702.06±2.16 ?g/mL). The total antioxidant capacity was higher in the acetone extract (37.09±1.04 ?g/mL), whereas the aqueous extract exhibited a higher reducing power. These findings suggested that the antioxidant activities in the different in vitro systems might be due to the presence of secondary metabolites and vitamin C in the A. polystachya leaves extract.