2025, Vol. 13, Issue 6, Part A

author(s)
Maitri M Thakor and Ashok H Patel

abstract
Herbal plants have long been used to treat various maladies. This study investigated the phytochemical constituents of Ocimum sanctum L. (stem and leaves) collected from the city area of Mehsana, Gujarat and subjected to extraction by maceration method with two different solvents viz. methanol and aqueous. Phytochemical estimation revealed that the methanolic extracts of O. sanctum L. yielded highest extractive value than aqueous and contained alkaloids, phenols, flavonoids, tannin etc. The methanolic extract of O. sanctum L. (leaves) yielded highest flavonoid content of 19.55±0.58 mg/g with quercetin. Antibacterial activity studied by disc diffusion method against S. aureus and E. coli bacteria, showing significant potential. These findings strongly supports its widespread use in folk medicine for various chronic disease. The results indicate that medicinally bioactive compounds possesses promising therapeutically value and could be further explore for formulation development and its application in foods, nutrition, pharmaceutical industries and human health.