The present ethnobotanical exploration documents about seventy six (76) different kinds of traditionally used medicinal plants in Bajali sub-division area, Barpeta, Assam. Out of these 76 phyto species 25% herbs, 34.21% shrub, 30.26% tree, 5.26% climber, 3.95% grass and 1.32% under tree grass category plants (Based on life forms). And maximum phyto species are used against different affliction, such as cough, diarrhoea, dysentery, jaundice, menstrual problem, piles, snakebite, diabetes, tonsillitis, blood purification, asthma, fever and skin diseases etc. The most important medicinal plant families were Asteraceae, Lamiaceae, Rutaceae, Apocynaceae, Araceae, Poaceae, Solanaceae and Piperaceae.