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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2015, Vol. 3, Issue 4, Part A
Protocol Establishment for Multiplication and Regeneration of ‘Holy Basil’ (Ocimum sanctum Linn). An Important Medicinal plant with High Religious Value in India

Tulika Mishra

Ocimum sanctum Linn, also known as “Holy Basil” is one of the most common medicinal plants used by diverse cultures and tribal groups. An efficient protocol for rapid propagation of O. sanctum, of the family Lamiaceae, was developed using leaf explants culture. The leaf explants cultured on Murashige and Skoog (MS) basal medium were supplemented with various concentrations and combinations of auxins and cytokinins. Callus induction was obtained within 7-10 days, Picloram at 3mg/l formed profuse callus and the degree was found to be the highest (+++++) among all the treatments. The best response to shoot induction, with maximum shoot elongation 6.8±0.14 was obtained using 1.0mg/l 6-benzyl aminopurine (BA) in combination with 0.5 mg/l Indole Acetic Acid (IAA). This medium showed 82% shoot bud proliferation with 23.8±0.23 mean number of shoots. In vitro shoots were rooted on 1.5mg/l of Indole Butyric Acid (IBA) supplemented medium. The rooted shoots were successfully acclimatized and established under natural conditions, with 90% survival rate.
Regenerated plants of O. sanctum maintained in green house on a potting mixture of 2:1 of compost and soil.
Fig.: Regenerated plants of O. sanctum maintained in green house on a potting mixture of 2:1 of compost and soil.
Pages : 16-19 | 1147 Views | 42 Downloads
How to cite this article:
Tulika Mishra. Protocol Establishment for Multiplication and Regeneration of ‘Holy Basil’ (Ocimum sanctum Linn). An Important Medicinal plant with High Religious Value in India. J Med Plants Stud 2015;3(4):16-19.
Journal of Medicinal Plants Studies
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