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Journal of Medicinal Plants Studies
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ISSN Print: 2394-0530, ISSN Online: 2320-3862, NAAS Rating: 3.53

NAAS Rating: 3.53 NEW

Journal of Medicinal Plants Studies

2016, Vol. 4, Issue 1, Part A
Curcumin reversion of neurochemical and immunohistochemical alterations in brain ischemia is related to its antioxidant and anti-inflammatory properties

Elaine Cristina Pereira Lucetti, Daniel Luna Lucetti, Ana Elisa da Silva Ribeiro, Rafael Barbosa de Moura, Thayga Maria Araújo Sampaio, Viviane Leandro de Almeida, Andreza Sérvula Pereira da Silva, Louise Rayna Alves Bezerra, Kelly Rose Tavares Neves, Glauce Socorro de Barros Viana

Stroke is one leading cause of mortality and morbidity worldwide where free radicals production and inflammation play important roles. Curcumin, a curcuminoid from Curcuma longa presents several biological properties and is a potent anti-inflammatory and antioxidant agent. The objectives were to study the neuroprotective effects of curcumin on ischemic pups from mothers treated with this drug (25, 50 or 100 mg/kg) during lactation. Twenty-one-day old pups were anesthetized with ketamine and xylazine, and submitted to global ischemia by clamping the carotid arteries, followed by 15 min reperfusion of the right artery and cut of the left artery. At the next day, the pups were treated with curcumin (25, 50 or 100 mg/kg) or vehicle, for 3 days. The sham operated group (SO) was subjected to the same procedure, except for carotid clamping. After 3 days, the animals were euthanized and brain areas isolated for neurochemical (monoamines, lipid peroxidation and nitrite determinations), histological (fluoro-jade staining) and immunohistochemical (TNF-alpha, iNOS and COX-2) assays. The results show that ischemia decreased DA and DOPAC as well as NE contents in the pups’ striatum, as related to the SO group, and curcumin treatments reversed these alterations. Brain ischemia decreased hippocampal neuronal viability also reversed by curcumin. Furthermore, ischemia drastically increased TNF-alpha, iNOS and COX-2 immunore activities in the hippocampus, and these effects were completely reversed after curcumin treatments. In conclusion, curcumin treatments reversed brain alterations presented after ischemia, representing a potential drug to be considered for prevention or treatment of pathological conditions as stroke.
Representative photomicrographs (magnification x400, scale bars=200 μm) of fluoro-jade staining in hippocampal CA1 and CA3 areas. The data were analyzed by the Image J software (NIH, USA). CA1: a. vs. SO, q=50.91; b. vs. ISC+C50, q=49.20; c. vs. ISC+C100, q=51.57; CA3: a. vs. SO, q=56.56; b. vs. ISC+C50, q=59.10; c. vs. ISC+C100, q=57.36. (One-way ANOVA and Tukey as the post hoc test).
Fig.: Representative photomicrographs (magnification x400, scale bars=200 μm) of fluoro-jade staining in hippocampal CA1 and CA3 areas. The data were analyzed by the Image J software (NIH, USA). CA1: a. vs. SO, q=50.91; b. vs. ISC+C50, q=49.20; c. vs. ISC+C100, q=51.57; CA3: a. vs. SO, q=56.56; b. vs. ISC+C50, q=59.10; c. vs. ISC+C100, q=57.36. (One-way ANOVA and Tukey as the post hoc test).
Pages : 20-29 | 1100 Views | 58 Downloads
How to cite this article:
Elaine Cristina Pereira Lucetti, Daniel Luna Lucetti, Ana Elisa da Silva Ribeiro, Rafael Barbosa de Moura, Thayga Maria Araújo Sampaio, Viviane Leandro de Almeida, Andreza Sérvula Pereira da Silva, Louise Rayna Alves Bezerra, Kelly Rose Tavares Neves, Glauce Socorro de Barros Viana. Curcumin reversion of neurochemical and immunohistochemical alterations in brain ischemia is related to its antioxidant and anti-inflammatory properties. J Med Plants Stud 2016;4(1):20-29.
Journal of Medicinal Plants Studies
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