Betel leaf (Piper betel L.) is an important plant being widely consumed worldwide as well as being used in traditional medicine. The present study aims to evaluate the anti-lipid peroxidation and anti-Candida potentials of different varieties of betel leaf extracts. Three widely consumed betel leaf varieties of India (Kolkata Variety, Varanasi Variety, and Mysore Variety) were used to evaluate anti-lipid peroxidation, and anti-Candida potentials in different extracts (ethyl acetate, acetone, and methanol). Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) were found to be higher in the ethyl acetate extract of all the varieties and hence selected for medicinal potential evaluation. The Mysore variety exhibited higher antioxidant and free radical scavenging properties IC50 value 179.90 ± 2.98 μg/mL and 319.52 ± 4.00 μg/mL respectively. The Varanasi variety with the IC50 value of 51.54 ± 1.81 μg/mL has slightly high anti-lipid peroxidation potentials over the Mysore variety (IC50 value 61.67 ± 2.92 μg/mL) over the standard butylated hydroxytoluene (BHT) with an IC50 value of 276.33 ± 15.20 μg/mL. The oral lotion disinfectant formulation showed anti-Candida activity in a dose-dependent fashion and the activity was higher than that of the standard drug fluconazole with the inhibition zone of 15mm over fluconazole having zone of inhibition of 12mm. The present study confirmed that the antioxidant and anti-lipid peroxidation potentials of the ethyl acetate extract of the Mysore and Varanasi variety respectively. The study also confirmed the anti-Candida activity of the ethyl acetate extract of betel leaf.